Michael Leibowitz talks about CRISPR gene editing for gene therapy applications can cause massive damage to chromosomes. The phenomenon is known as chromothripsis. The fact that the damage occurs “on-target” – at the intended edit site – means that any attempts to target the CRISPR gene editing more precisely will not solve this problem.
Leibowitz ML et al (2021). Chromothripsis as an on-target consequence of CRISPR-Cas9 genome editing. Nat Genet. 2021 Jun;53(6):895-905. doi: 10.1038/s41588-021-00838-7. Epub 2021 Apr 12. https://pubmed.ncbi.nlm.nih.gov/33846636/
CRISPR gene editing for gene therapy applications can cause massive damage to chromosomes. The phenomenon is known as chromothripsis. The fact that the damage occurs “on-target” – at the intended edit site – means that any attempts to target the CRISPR gene editing more precisely will not solve this problem.